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You are here:Home » Antibodies » Abs to Motor Proteins » Anti -KIF5B (Kinesin Family Member 5B, Kinesin 5B, Kinesin 1, Kinesin Heavy Chain, KINH, KNS1, Ubiquitous Kinesin Heavy Chain, UKHC)

Anti -KIF5B (Kinesin Family Member 5B, Kinesin 5B, Kinesin 1, Kinesin Heavy Chain, KINH,
KNS1, Ubiquitous Kinesin Heavy Chain, UKHC)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
KIF5B is a kinesin. These are microtubule based motor proteins involved in the transport of organelles in eukaryotic cells. They typically consist of 2 identical, approximately 110 to 120kD heavy chains and 2 identical, approximately 60 to 70kD light chains. The heavy chain contains 3 domains: a globular N terminal motor domain, which converts the chemical energy of ATP into a motile force along microtubules in 1 fixed direction; a central alpha helical rod domain, which enables the 2 heavy chains to dimerize; and a globular C terminal domain, which interacts with light chains and possibly an organelle receptor.
Catalog #K1759-24
ApplicationsSuitable for use in Peptide ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsPeptide ELISA: 1:1000
Western Blot: Detects a band at ~120kD in human kidney lysates
Immunohistochemistry (Paraffin; human kidney): 1-2ug/ml. Microwaved antigen retrieval with Tris/EDTA, pH 9. Shows perinuclear labeling of some glomerular cells and cytoplasmic staining of renal tubules.
Immunohistochemistry (paraffin; human uterus): 2-3ug/ml. Steamed antigen retrieval with citrate buffer, pH 6. Shows strong staining of the epithelium on endometrial glands.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa 951-963, QPVAVRGGGGKQV, from the C-terminus of human KIF5B (NP_004512). Cellular Localization: Uniformly distributed between the cell body and the processes in the neurons.
SpecificityRecognizes human KIF5B at ~120kD. Species Crossreactivity: canine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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