Anti -KIF5B (Kinesin Family Member 5B, Kinesin 5B, Kinesin 1, Kinesin Heavy Chain, KINH,
KNS1, Ubiquitous Kinesin Heavy Chain, UKHC)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B IH |
|
| KIF5B is a kinesin. These are microtubule based motor proteins involved in the transport of organelles in eukaryotic cells. They typically consist of 2 identical, approximately 110 to 120kD heavy chains and 2 identical, approximately 60 to 70kD light chains. The heavy chain contains 3 domains: a globular N terminal motor domain, which converts the chemical energy of ATP into a motile force along microtubules in 1 fixed direction; a central alpha helical rod domain, which enables the 2 heavy chains to dimerize; and a globular C terminal domain, which interacts with light chains and possibly an organelle receptor. | | | Catalog # | K1759-24 | | Applications | Suitable for use in Peptide ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Peptide ELISA: 1:1000 | | Western Blot: Detects a band at ~120kD in human kidney lysates | | Immunohistochemistry (Paraffin; human kidney): 1-2ug/ml. Microwaved antigen retrieval with Tris/EDTA, pH 9. Shows perinuclear labeling of some glomerular cells and cytoplasmic staining of renal tubules. | | Immunohistochemistry (paraffin; human uterus): 2-3ug/ml. Steamed antigen retrieval with citrate buffer, pH 6. Shows strong staining of the epithelium on endometrial glands. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to aa 951-963, QPVAVRGGGGKQV, from the C-terminus of human KIF5B (NP_004512). Cellular Localization: Uniformly distributed between the cell body and the processes in the neurons. | | Specificity | Recognizes human KIF5B at ~120kD. Species Crossreactivity: canine | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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