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You are here:Home » Antibodies » Abs to Motor Proteins » Anti -KIF5B (Kinesin-1 Heavy Chain, Conventional Kinesin Heavy Chain, Ubiquitous Kinesin Heavy Chain, UKHC, KNS, KNS1)

Anti -KIF5B (Kinesin-1 Heavy Chain, Conventional Kinesin Heavy Chain, Ubiquitous Kinesin
Heavy Chain, UKHC, KNS, KNS1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
KIF5B is a kinesin. These are microtubule based motor proteins involved in the transport of organelles in eukaryotic cells. They typically consist of 2 identical, approximately 110 to 120kD heavy chains and 2 identical, approximately 60 to 70kD light chains. The heavy chain contains 3 domains: a globular N terminal motor domain, which converts the chemical energy of ATP into a motile force along microtubules in 1 fixed direction; a central alpha helical rod domain, which enables the 2 heavy chains to dimerize; and a globular C terminal domain, which interacts with light chains and possibly an organelle receptor.
Catalog #K1759-24J
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:32,000
Western Blot: 0.5-1.5ug/ml, observed in lysates of the cell lines Jurkat and HeLa on ~110kD bands
Immunohistochemistry (Paraffin): 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-QPVAVRGGGGKQV, from KIF5B, at C-terminal (NP_004512.1).
SpecificityRecognizes human KIF5B. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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