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You are here:Home » Antibodies » Abs to Motor Proteins » Anti -KIF21A (Kinesin Family Member 21A)

Anti -KIF21A (Kinesin Family Member 21A)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #K1760-01
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml detects KIF21A in a rat brain microsomal preparation. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with K1760-01 (2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to amino acids 948-963 (CKRREKLSKRREKIVKE) of mouse KIF21A with an N-terminal cysteine added for conjugation purposes (KLH coupled).
SpecificityRecognizes mouse KIF21A, Mr ~180kD. An unknown protein detected at Mr ~70kD was also competed by the immunizing peptide. Species Crossreactivity: rat and human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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