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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IP IH IC RIA

Kinesin belongs to the group of motor proteins known to convert chemical energy released from ATP into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport inneuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120-130kD) and two light chains (60-70kD), resulting in a molecular mass about 400kD. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.
Catalog #	K1780-01
Applications	Suitable for use in ELISA, Immunohistochemistry, Immunocytochemistry, Western Blot, Immunoprecipitation and RIA. Other applications not tested.
Recommended Dilution	Immunohistochemistry: Paraformaldehyde fixed tissue. Also works for EM.
	Western Blot: 1:500 Kinesin (Heavy Chain) on 10ug mouse brain lysates. Mouse brain lystaes were resolved by electrophoresis, transferred to PVDF membrane and probed with K1780-01 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
	Optimal dilution determined by the researcher.
Positive Control	Brain tissue
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	2Q81
Host	Mouse
Source	Bovine
Concentration	~1mg/ml
Form	Supplied as a liquid in 0.02M PBS, pH 7.6, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Bovine brain kinesin
Specificity	Recognizes bovine heavy chain of kinesin near the N-terminus.
Species Crossreactivity	All mammals, chicken, squid, sea urchins and clams.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.