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You are here:Home » Antibodies » Abs to Motor Proteins » Anti -Kinesin, Heavy Chain

Anti -Kinesin, Heavy Chain

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH IC RIA
Kinesin belongs to the group of motor proteins known to convert chemical energy released from ATP into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport inneuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120-130kD) and two light chains (60-70kD), resulting in a molecular mass about 400kD. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.
Catalog #K1780-01
ApplicationsSuitable for use in ELISA, Immunohistochemistry, Immunocytochemistry, Western Blot, Immunoprecipitation and RIA. Other applications not tested.
Recommended DilutionImmunohistochemistry: Paraformaldehyde fixed tissue. Also works for EM.
Western Blot: 1:500 Kinesin (Heavy Chain) on 10ug mouse brain lysates. Mouse brain lystaes were resolved by electrophoresis, transferred to PVDF membrane and probed with K1780-01 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilution determined by the researcher.
Positive ControlBrain tissue
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No2Q81
HostMouse
SourceBovine
Concentration~1mg/ml
FormSupplied as a liquid in 0.02M PBS, pH 7.6, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenBovine brain kinesin
SpecificityRecognizes bovine heavy chain of kinesin near the N-terminus.
Species CrossreactivityAll mammals, chicken, squid, sea urchins and clams.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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