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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B IP IH IC RIA

Kinesin belongs to the group of motor proteins known to convert chemical energy released from ATP into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport inneuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120-130kD) and two light chains (60-70kD), resulting in a molecular mass about 400kD. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.
Catalog #	K1780-02
Applications	Suitable for use in Immunohistochemistry, Immunocytochemistry, Western Blot, ELISA, RIA and Immunoprecipitation. Other applications have not been tested.
Recommended Dilutions	Immunohistochemistry: Paraformaldehyde fixed tissue. Also works for EM.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	N/A
Clone Type	Monoclonal
Isotype	IgG1
Clone No	2Q80
Host	Mouse
Source	Bovine
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Purity	Purified
Immunogen	Bovine brain kinesin
Specificity	Recognizes the heavy chain of bovine kinesin near the N-terminus.
Species Crossreactivity	All mammals, other vertebrates not tested. Does not react with invertebrates.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.