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You are here:Home » Antibodies » Abs to Motor Proteins » Anti -Kinesin, Heavy Chain

Anti -Kinesin, Heavy Chain

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IP IH IC RIA
Kinesin belongs to the group of motor proteins known to convert chemical energy released from ATP into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport inneuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120-130kD) and two light chains (60-70kD), resulting in a molecular mass about 400kD. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.
Catalog #K1780-02
ApplicationsSuitable for use in Immunohistochemistry, Immunocytochemistry, Western Blot, ELISA, RIA and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry: Paraformaldehyde fixed tissue. Also works for EM.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q80
HostMouse
SourceBovine
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
PurityPurified
ImmunogenBovine brain kinesin
SpecificityRecognizes the heavy chain of bovine kinesin near the N-terminus.
Species CrossreactivityAll mammals, other vertebrates not tested. Does not react with invertebrates.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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