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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IP RIA

Kinesin belongs to the group of motor proteins known to convert chemical energy released from ATP into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport inneuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120-130kD) and two light chains (60-70kD), resulting in a molecular mass about 400kD. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.
Catalog #	K1782
Applications	Suitable for use in Western Blot, Immunopreciptiation, EIA and RIA. Not suitable for use in Immunohistochemistry or Immunocytochemistry. Other applications have not been tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	2Q82
Host	Mouse
Source	Bovine
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide before the addition of glycerol to 40%.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Bovine brain kinesin.
Specificity	Recognizes the light chain of kinesin within the last 50 amino acids from the N-Terminal. Species Crossreactivity: Crosseactive with most mammals.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.