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You are here:Home » Antibodies » Abs to Motor Proteins » Anti -Kinesin, Heavy Chain

Anti -Kinesin, Heavy Chain

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IC IF
Kinesin belongs to the group of motor proteins known to convert chemical energy released from ATP into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport inneuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120-130kD) and two light chains (60-70kD), resulting in a molecular mass about 400kD. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.
Catalog #K1782-20
ApplicationsSuitable for use in Western Blot, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Recommended DilutionWestern Blot: Only with kinesin-enriched preparations.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer..
Clone TypeMonoclonal
IsotypeIgM
Clone No4i354
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
PurityPurified by affinity chromatography
ImmunogenFull length native protein (purified) (Porcine).
SpecificityRecognizes porcine conventional kinesin associated with vesicles and with lower affinity with denaturated molecule. Recognizes the heavy chain of kinesin. No crossreaction with the light chain of the molecule nor with other proteins was detected. Species Crossreactivity: human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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