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You are here:Home » Antibodies » Abs to Motor Proteins » Anti -Kinesin, Heavy Chain, CT

Anti -Kinesin, Heavy Chain, CT

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Otherwise known as KIF5B, a member of the kinesin-like protein family and principal component of kinesin, involved in the plus end-directed transport of eukaryotic cell vesicles and organelles. Kinesins are microtubule associated motor proteins which consist of 2 identical (60-70kD) light chains and 2 identical (110-120kD) heavy chains, the latter of which contain a globular C-terminal tail domain, a central alpha helical rod domain and a globular N-terminal motor domain with microtubule-stimulated ATPase activity.
Catalog #K1782-20K
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Positive Control TissueHuman kidney
Recommended DilutionELISA: 1:32,000
Western Blot: 0.2-1.0ug/ml, detects a band of ~120kD in human kidney cell lysates.
Immunohistochemistry (Paraffin): 1-2ug/ml Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 is recommended for this purpose
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in TBS, pH7.3, 0.5% BSA, 0.02% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenPeptide sequence C-QPVAVRGGGGKQV from the C-terminal region of kinesin heavy chain (NP_004512.).
SpecificitySpecifically recognizes an epitope within the C-terminal (CT) region of human kinesin heavy chain (KHC). Speciessequence homology: canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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