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You are here:Home » Antibodies » Abs to Protease Inhibitors » Anti -Kininogen, Light Chain, High MW (HMWK)

Anti -Kininogen, Light Chain, High MW (HMWK)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B N
HMWK is one of the early participants of the intrinsic pathway of coagulation, together with Factor XII (Hageman factor) and prekallikrein. It is 626 amino acids long, and weighs 88 to 120kD (dependent on glycosylation). The kinigogen is not enzymatically active, and functions only as a cofactor for the activation of kallikrein and Hageman factor. It is also necessary for the activation of factor XI by factor XIIa. The histidine-rich region (amino acids 420 to 510) participates most strongly in coagulation. In addition to its role in blood coagulation, HMWK (just as Low-molecular-weight kininogen) is a strong inhibitor of cysteine proteinases. Responsible for this activity are three related domains on its heavy chain (Higashiyama et al., Biochemistry 25, 1669-75 (1986)).
HMWK is also a precursor of bradykinin; this vasodilator substance is released through positive feedback by kallikrein.
Catalog #K1801
ApplicationsSuitable for use in ELISA, Western Blot and Coagulation Assays. Other applications not tested.
Recommended DilutionDilute in PBS or medium which is identical to that used in the assay system.
Western Blot: 5.5% alkaline PAGE without detergents.
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No1.B.709
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No preservatives added.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified High molecular weight kininogen (HMWK).
SpecificityRecognizes the light chain (46kD) of human high MW kininogen and neutralizes the coagulant activity of high MW kininogen. Does not recognize kininogen that has been trested with SDS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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