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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -KLF15 (Kruppel-like Factor 15, Kidney-enriched Kruppel-like Factor, KKLF)

Anti -KLF15 (Kruppel-like Factor 15, Kidney-enriched Kruppel-like Factor, KKLF)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
The kidney chloride channel proteins CLCNKA and CLCNKB are highly related, although they are located in different areas of the kidney. Using a yeast 1-hybrid screen of a kidney cDNA library with the GA element of the CLCNKA promoter as bait, Uchida et al. (2000) isolated cDNAs encoding MAZ and KLF15, which they termed KKLF. Sequence analysis predicted that the 415-amino acid KLF15 protein, which is 84% identical to the rat Klf15 protein, contains 3 zinc finger motifs at its C terminus, N-terminal serine-rich stretches, and a central proline-rich segment. EMSA analysis confirmed that KLF15 and MAZ interact with the GA element of the CLCNKA promoter and showed that KLF15 binds with higher affinity and is a functional competitor of MAZ. Northern blot analysis revealed highest expression of a 2.5-kb KLF15 transcript in liver, followed by heart, skeletal muscle, and kidney. No expression was found in bone marrow or lymphoid tissues. Western blot analysis showed expression of a 50-kD protein. Immunohistochemical analysis detected nuclear expression of KLF15 in liver sinusoid stellate cells, subcapsular fibroblasts, and portal fibroblasts. KLF15 was also expressed in cardiac and skeletal muscle interstitial cells and in kidney inner medulla, glomeruli, and cortical interstitium. Immunofluorescence microscopy, however, demonstrated no colocalization of KLF15 with CLCNKA.
Catalog #K1891-70
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-3ug/ml; ~65-70kD band seen in Human Liver lysate. Note the higher than expected size is in agreement with previous Western results (Mol Cell Biol 2000 Oct; 20(19): 7319-7331).
Optimal dilutions to be determined by the researcher.
Positive Control  
Human Liver lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
PurityPurified from goat serum by ammonium sulfate precipitation followed by immunoaffinity chromatography.
ImmunogenPeptide with sequence VDHLLPVDENFSS, from N-Terminus of the protein sequence according to NP_054798.
SpecificityReacts with human KLF15. Expected to crossreact with Rat and Mouse due to sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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