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You are here:Home » Molecular Biology » MB-Enzymes, Glycosidase » Klotho beta, Recombinant, Human (Beta-klotho, BKL, BetaKlotho, Klotho beta-like Protein)

Klotho beta, Recombinant, Human (Beta-klotho, BKL, BetaKlotho, Klotho beta-like Protein)

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Specifications

Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue. Like Klotho, Klotho beta facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex. The Klotho beta mediated interaction of human FGF19 (mouse FGF15) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1). Klotho beta is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, upregulating adipocyte insulin­dependent glucose uptake. The 1043aa type I transmembrane protein is composed of a 51aa signal sequence, a 943aa extracellular domain (ECD) containing two glycosidase-like regions, a 21aa transmembrane domain, and 28aa intracellular tail. Since Klotho-related proteins lack critical active site Glu residues present in beta-glycosidases, it was initially unclear whether they were functional enzymes. However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho beta is also possible. The extracellular domain shares 79%, 87%, 87% and 67% identity with mouse, equine, canine and rat Klotho beta, respectively. The low identity with rat reflects aa discordance within rodent ECD.
Catalog #K1893-06
Murine myeloma cell line, NS0-derived Phe53-Leu997, with a C-terminal 10-His tag.
SDS-PAGE126kD, reducing conditions
ActivityMeasured by its binding ability in a functional ELISA. When 2ug/ml (100ul/well) of rhKlotho beta was immobilized onto a Ms x hKlotho beta coated plate, the concentration of rhFGF-21 that produces 50% of the optimal binding response was found to be approximately 3-18ng/ml.
Endotoxin Level 1EU/1ug (LAL)
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight110kD
SourceMurine myeloma cell line, NS0-derived
Purity~95% by SDS-PAGE, reducing conditions and visualized with silver stain.
FormSupplied as a liquid in PBS, pH 6.8, 0.1mM EDTA, 50% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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