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You are here:Home » Antibodies » Abs to Dickkopf Proteins » Anti -Kremen (Kremen protein 1, Kringle-containing Protein Marking The Eye And The Nose, Kringle Domain-containing Transmembrane Protein 1, Dickkopf Receptor, KREMEN1, KRM1)

Anti -Kremen (Kremen protein 1, Kringle-containing Protein Marking The Eye And The Nose,
Kringle Domain-containing Transmembrane Protein 1, Dickkopf Receptor, KREMEN1, KRM1)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified
Kremen (Kringle-containing protein marking the eye and the nose) proteins are type I transmembrane proteins that contain extracellular kringle, WSC and CUB domains and an intracellular region without any conserved motifs (1). Two related members, Kremen-1 and -2, have been identified. Kremens bind a subset of the secreted Dickkopf (Dkk) proteins (Dkk-1, -2 and -4) with high affinity to modulate the canonical Wnt signaling pathway that is transduced by the ternary receptor complex composed of Wnt, the seven-transmembrane domain receptor Frizzled, and the LDL-receptor-related protein 5/6 (LRP5/6) co-receptor (2, 3). Within the Dkk family, Dkk-1 and -4 bind directly to the LRP5/6 co-receptor to antagonize the canonical Wnt/b-catenin signaling pathway, but not the planar cell polarity (PCP) signaling pathway that does not involve LRP5/6 (4). In contrast, Dkk-3 has no effect on Wnt signaling and Dkk-2 can function either as an LRP agonist or antagonist, depending on whether the cell expresses Kremen (5). Kremen co-operates with Dkk to antagonize Wnt signaling via formation of a Kremen-Dkk-LRP ternary complex that triggers the internalization and clearance of the complex from the cell surface (3). All three extracellular domains but not the cytoplasmic region of a membrane anchored Kremen are needed for binding to the second cysteine-rich domain of Dkks (3). Mouse Kremen-1 cDNA encodes a 473aa glycosylated protein with a putative 19aa signal peptide, a 372aa extracellular domain, a 21aa transmembrane domain and a 60aa cytoplasmic domain. In the extracellular domain, it shares 92%, 41% aa sequence identity with human Kremen-1 and mouse Kremen-2, respectively. Mouse Kremen-1 is widely expressed in diverse embryonic (apical ectodermal ridge of the developing fore- and hindlimb buds, telencephalon and the first brachial arch, myotome and sensory tissues) and adult (lung, heart, kidney, skeletal muscle and testis) tissues (1).
Catalog #K5000-01B
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionELISA: 0.1-0.4ug/ml
ELISA (capture): 2-8ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2A
Clone No11n122
HostRat
SourceMouse
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS, Trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenRecombinant corresponding to aa20-395 from Kremen-1 expressed in NSO cells (Q99N43).
SpecificityRecognizes mouse Kremen-1. Species sequence homology: human (92%). No cross-reactivity or interference with recombinant human Kremen- 2, recombinant mouse Kremen-2, Dkk-1, Dkk-4, or LRP-6.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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