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You are here:Home » Antibodies » Abs to Dickkopf Proteins » Anti -Kremen2

Anti -Kremen2

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
The Wnt family of secreted glycoproteins mediate cell-cell interaction during cell growth and differentiation in both embryos and adults. (1,2) Canonical Wnt signaling by way of the b-catenin pathway is transduced by two receptor families, frizzled proteins and lipoprotein-receptor-related proteins 5 and 6 (LRP5/6) which binds Wnts and transmit their signal by stabilizing intracellular b-catenin.(3-6) Kremen1 and Kremen2 are high-affinity Dkk1 receptors that functionally cooperate with the secreted protein Dickkopf1 (Dkk1) to block Wnt/b-catenin signaling.(7) Kremen1 and Kremen2 are components of a membrane complex modulating canonical Wnt signaling through LRP6 in vertebrates.(7) Dkk1 has been shown to inhibit Wnt signaling by binding to and antagonizing LRP5/6 (8-10).
Catalog #K5002
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.125mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide derived from the internal region of human, mouse and rat Kremen2 proteins.
SpecificityRecognizes human, mouse and rat Kremen2 proteins at ~51kD. No cross-reactivity with the related Kremen1 protein. Reactivity has been confirmed with myc-tagged mouse Kremen2-transfected 293T cell lysates. Species sequence homology: human and rat-100%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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