Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -KSR (Kinase Suppressor of Ras, KSR1, BKSR1, dKsr, EK31, hb, Positive Ras Signaling Mediator Family Member, RSU2, Suppressor of Activated let 60 Ras SUR3, Suppressor of Ras1 31, SR31)

Anti -KSR (Kinase Suppressor of Ras, KSR1, BKSR1, dKsr, EK31, hb, Positive Ras Signaling
Mediator Family Member, RSU2, Suppressor of Activated let 60 Ras SUR3, Suppressor of Ras1
31, SR31)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
KSR was initially considered to be a protein kinase acting in the Ras pathway, but has more recently been demonstrated to act as a scaffold protein, facilitating complex formation between RAF and MEK and ERK.
Catalog #K6850
ApplicationsSuitable for use in Immunoprecipitation and Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: Endogenous levels of KSR may be too low to detect by Western Blot. Immunoprecipitation prior to Western Blot is recommended for detection of endogenous KSR. Note levels of recombinant KSR in transfected cells may be high enough to be detected by Western Blot.
Immunoprecipitation/Western Blot: 4ug/ml detects KSR by Western Blot after using 10ug of this lot to immunoprecipitate KSR from 1mg of 3T3/A31 RIPA lysate. 3T3/A31 cell lysate was immunoprecipitated with 10ug of K6850 and immune complexes were resolved by electrophoresis, transferred to nitrocellulose and probed with K6850 (1:250). Proteins were visualized using an IgG(HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system. Other bands correspond to light chain, heavy chain, and heavy chain dimer. The band above KSR may be incompletely reduced antibody, as it was present in a nonspeci c antibody control.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG
Clone No4i45
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenGST fusion protein corresponding to residues 118-248 of rat KSR.
SpecificityRecognizes rat KSR, Mr 120kD. Species Crossreactivity: human and mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links