| The kidneys play a major role in the regulation of glucose levels. Kidneys filter approx. 180 g of glucose per day from the blood, and this is mostly reabsorbed back into the blood in the proximal tubules. Typically, glucose is first absorbed within epithelium by a specific transporter protein, Sodium glucose co- transporters (SGLT), in the brush-border membrane and then it is transported out of the cell across the basolateral membranes by a facilitated sugar transporter (GLUTs). At least 3 members of SGLTs (SGLT1-3) have been cloned and characterized from various species. Individual member of this family have identical predicted secondary structures with up to 14 transmembrane domains. SGLT1-3 genes code for protein of approx 659-672 residues (calculated size of ~75 kD). Both N and C-termini are predicted to be extracellular. |
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| A human gene, KST1, encoding a new member of SGLT family has been identified. KST1 (rabbit 674 aa, human 675 aa chromosome 16p12-p11), an ortholog of rabbit kidney SGLT or rkST, is expressed in brain, heart, muscle, kidney, liver and placenta. Due to its wide tissue expression and chromosomal location, mutations in KST1 have been implicated in ICCA (infantile convulsion and choreoathetosis) and BFIC (benign familial infantile convulsions). |
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| Catalog # | K6890-05 |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 1-10ug/ml using ECL. A band of 95-110kD has been detected in brush border membrane (2). |
| ELISA: 0.5-1ug/ml Control peptide can be used to coat ELISA plates at 1ug/ml. |
| Optimal dilutions to be determined by the researcher. |
| Control Peptide | K6890 KST1, Human Control Peptide |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4 and 0.1% BSA, 40% glycerol. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic 15aa peptide sequence from the N-terminal, cytoplasmic domain of Human KST1(KLH). |
| Specificity | Recognizes Human KST1. No significant sequence homology exists with other SGLTs. Species sequence homology: rabbit 66%. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
