| The Ku autoantigen is a heterodimer of 70kD (p70) and ~80kD (p80) proteins. The p70/p80 dimer is important for function of a 460kD DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis. |
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| Catalog # | K7965-05 |
| Applications | Suitable for use in Immunoprecipitation, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Not suitable for Western Blot. Optimal dilutions to be determined by the researcher. |
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| Recommended Dilution: |
| Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues. |
| Immunoprecipitation: Tested only on mouse and rat |
| Optimal dilutions to be determined by the researcher. |
| Recommended Positive Control | Tonsil |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 3F247 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.2mg/ml |
| Form | Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See K7965-05X |
| Purity | Purified by ammonium sulfate precipitation. |
| Immunogen | Human B cell nuclei from plasmacytoid 2p68 cells. Cellular Localization |
| Specificity | Recognizes human Ku, p70, p80. Species Crossreactivity: monkey, and xenopus. Mouse and rat (Immunoprecipitation only). Does not react with bovine and rabbit. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
