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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -Ku, p70, p80

Anti -Ku, p70, p80

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IP IH FC IF
The Ku autoantigen is a heterodimer of 70kD (p70) and ~80kD (p80) proteins. The p70/p80 dimer is important for function of a 460kD DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Catalog #K7965-05
ApplicationsSuitable for use in Immunoprecipitation, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Not suitable for Western Blot. Optimal dilutions to be determined by the researcher.
Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
Immunoprecipitation: Tested only on mouse and rat
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlTonsil
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3F247
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See K7965-05X
PurityPurified by ammonium sulfate precipitation.
ImmunogenHuman B cell nuclei from plasmacytoid 2p68 cells. Cellular Localization
SpecificityRecognizes human Ku, p70, p80. Species Crossreactivity: monkey, and xenopus. Mouse and rat (Immunoprecipitation only). Does not react with bovine and rabbit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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