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You are here:Home » Antibodies » Abs to Ribosomal Proteins » Anti -L11 Ribosomal Protein

Anti -L11 Ribosomal Protein

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Interactions between ribosomal protein L11 and a domain of large subunit rRNA have been highly conserved across species and are essential for efficient protein synthesis. In E. coli, L11 protein is located at the base of the L7/L12 stalk of the 50S subunit. The C-terminal domain of L11, L11-C76, recognizes and binds in the distorted minor groove of a helix within a 58-nucleotide domain of 23 S ribosomal RNA, whose secondary structure consists of three helical stems and a central junction loop. The conserved structural core of the protein consists of a bundle of three alpha-helices and a two-stranded parallel beta-sheets. When fused with His-Tag and S-tag sequences, L11 showed a molecular weight of around 21kD as expected.
Recently, L11 has been described as interacting with the human homolog of MDM2, HDM2, at a region distinct from the ARF binding site. L11 binding to HDM2 inhibits HDM2 function, leading to the stabilization and activation of p53.
Catalog #L0011
ApplicationsSuitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6A104
HostMouse
SourceHuman
Concentration0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenL11-GST fusion protein
SpecificitySpecific for endogenous human L11 proteins. On Western blots, it identifies a band at ~20kD. Reactivity has been confirmed with HEK293T cell lysates.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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