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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH IC

The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase; modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein.
Catalog #	L0899
Applications	Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions	Western Blot: 0.5-2ug/ml. Detects LacI from LacI transfected Cos-1 whole cell lysate.
	Immunocytochemistry: Reported to show nuclear immunostaining in LacI transfected cells fixed with methanol.
	Immunohistochemistry: Reported to show nuclear immunostaining in LacI transgenic tissue fixed with 4% paraformaldehyde and permeabilized with Triton X-100.
	Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies	I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
	I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	0.T.81
Host	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.0, 0.01M EDTA.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant TrpE-fusion protein containing full length E. coli LacI.
Specificity	Recognizes E. coli LacI at 38kD and recombinant proteins containing a LacI tag.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.