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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » D-Lactate Assay Kit, BioAssay™, Colorimetric

D-Lactate Assay Kit, BioAssay™, Colorimetric

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Specifications

D-Lactate production in mammals, mainly due to the glyoxalase pathway, is extremely low,
with normal serum concentrations in the nano to micromolar range. Typically, elevated D-lactate
levels which can rise to millimolar levels, are due to bacterial infection or short bowel syndrome in humans. Abnormally high concentrations of D-lactate are considered indicative of sepsis, ischemia or trauma. Due to slow metabolism and excretion, high D-lactate can cause acidosis and encephalopathy.
Catalog #L1018-01
Intended UseThe D-Lactate Assay Kit provides a fast, easy way to accurately measure D-lactate in a variety of biological samples. In the D-Lactate Assay Kit, D-lactate is specifically oxidized by D-lactate dehydrogenase and generates proportional color (lambda-max = 450nm). The kit detects D-Lactate in samples such as serum, plasma, cells, culture and fermentation media. The useful concentration range in samples is 0.01mM--10mM Dlactate.
Kit ComponentsD-Lactate Assay Buffer 1x25ml
D-Lactate Enzyme Mix lyophilized
D-Lactate Substrate Mix lyophilized
D-Lactate Standard (100mM) 1x100ul.
Storage and StabilityStore the kit at -20°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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