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You are here:Home » Antibodies » Abs to Serum Proteins » Anti -Lactoferrin (Biotin)

Anti -Lactoferrin (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH IC FC IF
Lactoferrin is an iron binding glycoprotein with an approximate molecular weight of 80kD. The protein has two iron binding domains each housing one Fe 3+ and the synergistic CO 3 2- ion. The crystal structure form of human lactoferrin at 2.2A resolution exhibits 5330 protein atoms, 2Fe2+, 2CO 32- and 98 carbohydrate atoms. Lactoferrin is absorbed from intestine by apical side of the membrane and localized to the nuclei. Intravenous infusion of lactoferrin is protective against lethal doses of E. coli and induce bacterimia by a mechanism that downregulates neutrophil TNF alfa secretion. Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency induced anemia. Lactoferrin has been shown to have a role in the immune system and in early development of the embryo. A specific receptor for lactoferrin binding has been implicated in the human fetal intestine. Early embryonic localization of lactoferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain.
Catalog #L1021-77
ApplicationsSuitable for use in ELISA, Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, and Immunofluorescence. Other applications not tested.
Recommended DilutionsELISA: 1:20,000-1:400,000 using enzyme-conjugated streptavidin
Western Blot: 1:20,000-1:400,000 using enzyme-conjugated streptavidin
Immunohistochemistry (Enzymatic): 1:500-1:5000 on tissue sections
Flow Cytometry: 1:200-1:1000
Fluorescence Immunohistochemistry/Immunocytochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 15mg/ml BSA, 0.05% sodium azide. Labeled with Biotin.
PurityPurified by immunoaffinity chromatography.
ImmunogenLactoferrin purified from human colostrum
SpecificityRecognizes human lactoferrin. Does not crossreact with other milk or serum proteins. Species crossreactivity: May crossreact with lactoferrin from other species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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