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You are here:Home » Antibodies » Abs to IgG Lambda » Anti -Lambda Light Chain

Anti -Lambda Light Chain

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IH
Lambda light chains are one of two types of immunoglobulin light chains present in all five immunoglobulin classes. This antibody is useful for detecting lambda light chains found on the surface of normal and neoplastic B cells in lymphoid tissues. No reactivity has been observed with the similar kappa light chain. This antibody may also stain extracellular immunoglobulins.
Clonality of a given B cell population can be inferred from the uniformity of light chain class expression. A B cell can express either kappa or lambda light chains, but not both. The ratio of kappa: lambda bearing B cells being 2:1. A vast predominance of kappa or lambda light chain bearing B cells indicates monoclonality and generally implies a neoplastic proliferation. Polyclonality is suggested by a mixture of light chain bearing cell types and indicates a reactive or non- neoplastic proliferation of B cells.1
Catalog #L1185-03A
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry: 1:50-1:200. Frozen and formalin-fixed, paraffin-embedded tissue sections. Epitope Retrieval: Not Required Enzyme Digestion: Trypsin digestion is required.
Optimal dilutions to be determined by the researcher.
Positive Control Tonsil/ lymph node
Expected Staining Pattern Membrane
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20°C. Aliquots are stable for at least 6months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No6A105
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4, with 1% BSA with 0.05% sodium azide
PurityPurified from mouse ascites
ImmunogenHuman serum
SpecificityRecognizes human lambda light chains. No reactivity has been observed with the similar kappa light chain.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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