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You are here:Home » Antibodies » Abs to IgG Lambda » Anti -Lambda Light Chain

Anti -Lambda Light Chain

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified IH
Catalog #L1185-05
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (frozen & formalin/paraffin): 1-2ug/ml for 30 mins. at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Cellular Localization Cytoplasmic.
MW of Antigen 25kD.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No0.N.379
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide.
Also available without BSA and azide. See L1185-05X.
PurityPurified by Protein A affinity chromatography.
ImmunogenPurified human IgG myeloma proteins covalently coupled to polyaminostyrene micro beads.
SpecificityRecognizes the lambda light chains of human immunoglobulins. Also recognizes free and Bence-Jones lambda light chains. Species Reactivity: Human. Does not react with Rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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