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Specifications

Clone Host Grade Applications Monoclonal Mouse Highly Purified IH

Catalog #	L1185-05
Applications	Suitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Immunohistochemistry (frozen & formalin/paraffin): 1-2ug/ml for 30 mins. at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.
	Optimal dilutions to be determined by the researcher.
Cellular Localization	Cytoplasmic.
MW of Antigen	25kD.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	0.N.379
Host	Mouse
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide.
Also available without BSA and azide. See L1185-05X.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Purified human IgG myeloma proteins covalently coupled to polyaminostyrene micro beads.
Specificity	Recognizes the lambda light chains of human immunoglobulins. Also recognizes free and Bence-Jones lambda light chains. Species Reactivity: Human. Does not react with Rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.