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Anti -Lambda Light Chain (BSA & Azide Free)
Anti -Lambda Light Chain (BSA & Azide Free)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IH |
|
| Antibody to the lambda light chain of immunoglobulin is reportedly useful in the identification of leukemias, plasmacytomas and certain non-Hodgkin’s lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. | | | Catalog # | L1185-05X | | Applications | Suitable for use in Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunohistochemistry (Formalin/Paraffin Sections): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. | | Optimal dilutions to be determined by the researcher. | | Positive Control | Tonsil | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a,k | | Clone No | 0.N.379 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See L1185-05. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Purified human IgG myeloma proteins covalently coupled to polyaminostyrene microbeads. Cellular Localization | | Specificity | Recognizes the lambda light chains of human immunoglobulins. Also recognizes free and Bence-Jones lambda light chains. Does not crossreact with kappa light chains. Species Crosseactivity: Does not react with rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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