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You are here:Home » Antibodies » Abs to IgG Lambda » Anti -Lambda Light Chain (BSA & Azide Free)

Anti -Lambda Light Chain (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH
Antibody to the lambda light chain of immunoglobulin is reportedly useful in the identification of leukemias, plasmacytomas and certain non-Hodgkin’s lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Catalog #L1185-05X
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin/Paraffin Sections): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlTonsil
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No0.N.379
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See L1185-05.
PurityPurified by Protein A affinity chromatography.
ImmunogenPurified human IgG myeloma proteins covalently coupled to polyaminostyrene microbeads. Cellular Localization
SpecificityRecognizes the lambda light chains of human immunoglobulins. Also recognizes free and Bence-Jones lambda light chains. Does not crossreact with kappa light chains. Species Crosseactivity: Does not react with rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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