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You are here:Home » Antibodies » Abs to IgG Lambda » Anti -Lambda Light Chain

Anti -Lambda Light Chain

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified IH
This antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single light chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Catalog #L1185-07
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin): 1:100-1:200 for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control Tonsil
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 24 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined.
FormSupplied as liquid in PBS, pH 7.4, 0.09% sodium azide, 0.2% BSA. Also supplied without BSA and sodium azide. See L1185-07X.
PurityPurified
ImmunogenLambda light chains purified from human Bence Jones proteins. Cellular Localization
SpecificityRecognizes lambda light chains of human immunoglobulins, free lambda light chains and Bence-Jones lambda light chains (25kD). Does not crossreact with kappa light chains. Species Crossreactivity: Human, baboon, mouse and rat. Does not crossreact with canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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