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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E FC

Catalog #	L1185-11D
Applications	Suitable for use in Flow Cytometry and ELISA. Other applications not tested.
Recommended Dilutions	Flow Cytometry: 1:5-1:10. Use 10ul to label 10e6 cells or 100ul washed whole blood.
	Optimal working dilutions to be determined by researcher.
Hybridoma	Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Recommended Negative Control	I1904-78R: IgG1 Negative Control
Recommended Secondary Antibody	I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb xMo
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H2592
Host	Mouse
Source	Human
Concentration	~0.25mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, containing 0.09% sodium azide, before the addition of glycerol to 40%.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Purified human IgG.
Specificity	Recognizes human lambda light chains and shows no cross- reactivity to kappa light chains or to immunoglobulin heavy chains.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.