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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH IF

Laminin is a major component of basement membranes and stimulates neurite outgrowth, promotes cell attachment, chemotaxis and cell differentation. Promotes the adhesion and growth of human carcinoma and sarcoma cells, human retinoblastoma cells, liver cells, mouse neuroblastoma cells, mouse embryonal carcinoma cells, and seems to be involved in the development of embryonal tissue.
Catalog #	L1219-91
Applications	Suitable for use in Immunofluorescence Microscopy, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution	Immunohistochemistry (Frozen): 1:10 incubating for 1 hour at RT.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5F313
Host	Mouse
Source	Xenopus
Concentration	~0.05mg/ml
Form	Supplied as a lyophilized powder, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells). Epitope: Localized to coil 1B.
Specificity	Recognizes xenopus Lamin II and III. Reacts with lamin isotypes of Mr 60-75kD. Decorates the karyoskeleton (the intermediate filament equivalent of the nucleus). Species Crossreactivity: Trout, rat kangaroo (LII, LIII), bovine, human (LB1, B2), mouse (LB2), rat (L).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.