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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Lamin

Anti -Lamin

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Laminin is a major component of basement membranes and stimulates neurite outgrowth, promotes cell attachment, chemotaxis and cell differentation. Promotes the adhesion and growth of human carcinoma and sarcoma cells, human retinoblastoma cells, liver cells, mouse neuroblastoma cells, mouse embryonal carcinoma cells, and seems to be involved in the development of embryonal tissue.
Catalog #L1219-91
ApplicationsSuitable for use in Immunofluorescence Microscopy, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (Frozen): 1:10 incubating for 1 hour at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F313
HostMouse
SourceXenopus
Concentration~0.05mg/ml
FormSupplied as a lyophilized powder, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
PurityPurified by Protein A affinity chromatography.
ImmunogenNuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells). Epitope: Localized to coil 1B.
SpecificityRecognizes xenopus Lamin II and III. Reacts with lamin isotypes of Mr 60-75kD. Decorates the karyoskeleton (the intermediate filament equivalent of the nucleus). Species Crossreactivity: Trout, rat kangaroo (LII, LIII), bovine, human (LB1, B2), mouse (LB2), rat (L).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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