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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Lamin A

Anti -Lamin A

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH FC
Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization (1,3). Lamin A is cleaved by caspase-6 and serves as a marker for caspase- 6 activation. During apoptosis, the 70 kD lamin A is specifically cleaved to a large (40-45 kD) and a small (28 kD) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).
Catalog #L1220-01
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionsWestern Blot 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:200
Flow Cytometry: 1:50
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A and immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to residues surrounding Asp230 of human lamin A (KLH).
SpecificityRecognizes human Lamin A. Detects total levels of endogenous full length lamin A and lamin C (70kD), as well as the small fragment of lamin A (and the lamin C isotype) resulting from cleavage at Asp230 (28kD). Species Crossreactivity: human, mouse and rat..
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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