Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP IH FC

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization (1,3). Lamin A is cleaved by caspase-6 and serves as a marker for caspase- 6 activation. During apoptosis, the 70 kD lamin A is specifically cleaved to a large (40-45 kD) and a small (28 kD) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).
Catalog #	L1220-01
Applications	Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended Dilutions	Western Blot 1:1000
	Immunoprecipitation: 1:50
	Immunohistochemistry (Paraffin): 1:200
	Flow Cytometry: 1:50
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	As reported
Form	Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity	Purified by Protein A and immunoaffinity chromatography.
Immunogen	Synthetic peptide corresponding to residues surrounding Asp230 of human lamin A (KLH).
Specificity	Recognizes human Lamin A. Detects total levels of endogenous full length lamin A and lamin C (70kD), as well as the small fragment of lamin A (and the lamin C isotype) resulting from cleavage at Asp230 (28kD). Species Crossreactivity: human, mouse and rat..
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.