Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Lamin A,C

Anti -Lamin A,C

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IH IC
Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished: A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Type A lamins comprise a set of three proteins arising from the same gene by alternative splicing: lamins A, C, and Adel 10. B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Mutations in A-lamins give rise to a range of genetic disorders, including autosomal dominant Emery-Dreifuss muscular dystrophy (AD-EDMD), dilated cardiomyopathy and Dunnigan-type familial partial lipodystrophy. Aberrant expression patterns of nuclear lamins have been described in various types of cancer depending on the subtype of cancer, its aggressiveness, proliferative capacity and degree of differentiation. In general, the expression of A-type lamins (lamins A and C) has been correlated with a non-proliferating, differentiated state of cells and tissues. Lamins A and C, the products of the LMNA gene, are nuclear intermediate filament proteins and are the major structural components of the lamina network that underlies and supports the nuclear envelope. Expression of A-lamin coincides with cell differentiation and may be related proteinís abnormal expression and organization in neoplastic tissues and cells.
Catalog #L1220-12
ApplicationsSuitable for use in Immunoblot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunoblot: 1:50-1:500
Immunocytochemistry: 1:50-1:100 (Cells fixed with methanol/acetone).
Immunohistochemistry: 1:50-1:100 (Frozen tissue sections).
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q1131
HostMouse
SourceRat
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide, before the addition of glycerol to 40%.
PurityPurified culture supernatant.
ImmunogenLamin purified from rat liver.
SpecificityRecognizes lamin A and C. Recognizes an epitope between aa319-566. Species Crossreactivity: human, rat, mouse, bovine, canine, sheep and hamster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links