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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B IC FC

Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.
Catalog #	L1220-21A
Applications	Suitable for use in Immunocytochemistry, Flow Cytometry, ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Immunocytochemistry: 1:100-1:200 with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
	Flow Cytometry: 1:100-1:200
	Western Blot: 1:100-1:1000
	Optimum dilutions to be determined by researcher.
Hybridoma	P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1k
Clone No	4A60
Host	Mouse
Source	Rat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 0.09% sodium azide. No stabilizing proteins added.
Purity	Purified
Immunogen	Purified Lamin B1 from rat liver
Specificity	Recognizes rat lamin B1. Reacts with an epitope located C-terminal to residue 231. Species Crossreactivity: human, mouse, bovine, rabbit, canine and sheep.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.