| Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished: A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Type A lamins comprise a set of three proteins arising from the same gene by alternative splicing: lamins A, C, and Adel 10. B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Mutations in A-lamins give rise to a range of genetic disorders, including autosomal dominant Emery-Dreifuss muscular dystrophy (AD-EDMD), dilated cardiomyopathy and Dunnigan-type familial partial lipodystrophy. Aberrant expression patterns of nuclear lamins have been described in various types of cancer depending on the subtype of cancer, its aggressiveness, proliferative capacity and degree of differentiation. In general, the expression of A-type lamins (lamins A and C) has been correlated with a non-proliferating, differentiated state of cells and tissues. Lamins A and C, the products of the LMNA gene, are nuclear intermediate filament proteins and are the major structural components of the lamina network that underlies and supports the nuclear envelope. Expression of A-lamin coincides with cell differentiation and may be related protein’s abnormal expression and organization in neoplastic tissues and cells. |
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| Catalog # | L1220-35 |
| Applications | Suitable for use in Western Blot, Immunocytochemistry and Flow cytometry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:100-1:1000 |
| Immunocytochemistry: 1:100-1:200 fixed cells (methanol/acetone fixation) |
| Flow Cytometry: 1:100-1:200 |
| Optimal dilution determined by the researcher. |
| Storage and Stability: |
| May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 2Q1130 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS with 0.1% sodium azide. |
| Purity | Purified culture supernatant |
| Immunogen | Detergent insoluble fraction of potoroo cell line PtK1. |
| Specificity | Recognizes an epitope located in the C-terminal part of lamin B2. Species Crossreactivity: human, mouse, hamster and Xenopus. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
