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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

Laminin is a large (900kD) complex extracellular glycoprotein and a major component of the basement membrane. Laminin is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin is composed of three different polypeptide chains; alpha, beta and gamma which are bound to each other by disulphide bonds into a cross-shaped molecule. Isoforms have been detected for all three chains. The different combinations of the alpha, beta and gamma chains give rise to at least 15 laminin heterotrimers.
Catalog #	L1225-01H
Applications
	Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 0.25-0.5ug/ml
	Immunohistochemistry (Paraffin and Formalin): 0.5-1ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	10g45
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a lyophilized powder in 1.2% sodium acetate, 0.2% sodium azide, 2% BSA. Reconstitute with 1ml sterile PBS (pH 7.4) to 100ug/ml.
Purity	Purified by affinity chromatography.
Immunogen	Human Laminin
Specificity	Recognizes human Laminin. Species Crossreactivity: feline, porcine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.