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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Laminin

Anti -Laminin

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
Catalog #L1225-06
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:2000-1:4000.
Western Blot: 1:250-1:1000
Immunohistochemistry (paraffin embedded sections): 1:25-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml (UV280)
FormSupplied as a liquid in 125mM sodium borate, 75mM sodium chloride, 5mM EDTA, pH 8.0, 0.01% sodium azide, before the addition of 40% glycerol.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to regions of adherence on laminin.
SpecificityRecognizes human laminin. Typically less than 1% crossreactivity against other extracellular matrix proteins was detected by ELISA against purified standards. Recognizes most mammalian Laminins and has negligible crossreactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific crossreaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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