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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum B IH

Laminin is a glycoprotein (Mr 850-1,000kD, consisting of 3 glycosylated polypeptide chains with molecular weights of 440 and 225 (2x) kD) produced by various human epithelial and
mesenchymal cells, and forms an extracellular matrix of thin filaments. In normal tissues, laminin is invariably present in all basal laminas surrounding muscle, nerve, fat and decidua cells and separates epithelial and endothelial cells from abutting connective tissues. Laminin has also been identified within the cytoplasm of breast epithelia, stromal cells of the endometrium, and within endothelial, bile duct epithelial and mesenchymal cells of the liver. Laminin has been found to be involved in cellular activities such as adhesion, spreading, differentiation, polarization, proliferation, locomotion, tissue invasion and chemotactic responses.
Catalog #	L1225-09
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions	Western Blot: 1:50-1:100
	Immunohistochemistry: 1:50-1:00 Frozen and paraffin tissues (proteolytic treatment required; enzyme: pepsin)
	Optimal dilutions to be determined by the researcher.
Positive Control	Skin, kidney
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	Not Determined
Form	Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide
Purity	Serum
Immunogen	Mouse laminin (laminin 1) derived from EHS sarcoma.
Specificity	Recognizes mouse laminin. Reacts with a distinct band of~200-220kD from a 8M Urea extract from amnion basement membrane. It does not crossreact with human type 1, III, IV and V collagen in Western Blot. Species Crossreactivity: human, bovine, porcine, rat, rabbit and most mammals.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.