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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Laminin

Anti -Laminin

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IH
Laminin is a glycoprotein (Mr 850-1,000kD, consisting of 3 glycosylated polypeptide chains with molecular weights of 440 and 225 (2x) kD) produced by various human epithelial and
mesenchymal cells, and forms an extracellular matrix of thin filaments. In normal tissues, laminin is invariably present in all basal laminas surrounding muscle, nerve, fat and decidua cells and separates epithelial and endothelial cells from abutting connective tissues. Laminin has also been identified within the cytoplasm of breast epithelia, stromal cells of the endometrium, and within endothelial, bile duct epithelial and mesenchymal cells of the liver. Laminin has been found to be involved in cellular activities such as adhesion, spreading, differentiation, polarization, proliferation, locomotion, tissue invasion and chemotactic responses.
Catalog #L1225-09
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:50-1:100
Immunohistochemistry: 1:50-1:00 Frozen and paraffin tissues (proteolytic treatment required; enzyme: pepsin)
Optimal dilutions to be determined by the researcher.
Positive ControlSkin, kidney
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot Determined
FormSupplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide
PuritySerum
ImmunogenMouse laminin (laminin 1) derived from EHS sarcoma.
SpecificityRecognizes mouse laminin. Reacts with a distinct band of~200-220kD from a 8M Urea extract from amnion basement membrane. It does not crossreact with human type 1, III, IV and V collagen in Western Blot. Species Crossreactivity: human, bovine, porcine, rat, rabbit and most mammals.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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