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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Laminin

Anti -Laminin

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum IH
The extracellular matrix (ECM) is a complex three-dimensional scaffold, which surrounds and separates most tissue compartments, determines tissue architecture and dynamically interacts with its surrounding cells. The basement membrane is a part of the ECM and is composed of collagens, proteoglycans, elastin and several glycoproteins of which laminin is the most abundant. Antibody labels basement membrane surrounding normal vessels, glands, muscles, nerves, fat and deciduas cells separating these from abutting connective tissues.
Catalog #L1225-10
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin/ paraffin): 1:25-1:50. Incubate for 30 min at RT . For staining of formalin-fixed tissues, digest sections with Protease XXV at 1mg/ml PBS for 5 minutes at 37°C.
Optimal dilutions to be determined by the researcher.
Positive ControlSkin, Lung, Kidney or Tonsil.
Cellular LocalizationBasement membrane.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceRat
ConcentrationNot determined.
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 0.2% BSA, before the addition of glycerol 40%. Also supplied without BSA and Sodium Azide. See L1225-10X.
PurityConcentrated antisera
ImmunogenLaminin isolated from a rat yolk sac tumor cell line. Cellular Localization: Basement membrane.
SpecificityRecognizes rat Laminin. Species crossreactivity: Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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