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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum IH

The extracellular matrix (ECM) is a complex three-dimensional scaffold, which surrounds and separates most tissue compartments, determines tissue architecture and dynamically interacts with its surrounding cells. The basement membrane is a part of the ECM and is composed of collagens, proteoglycans, elastin and several glycoproteins of which laminin is the most abundant. Antibody labels basement membrane surrounding normal vessels, glands, muscles, nerves, fat and deciduas cells separating these from abutting connective tissues.
Catalog #	L1225-10
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry (Formalin/ paraffin): 1:25-1:50. Incubate for 30 min at RT . For staining of formalin-fixed tissues, digest sections with Protease XXV at 1mg/ml PBS for 5 minutes at 37°C.
	Optimal dilutions to be determined by the researcher.
Positive Control	Skin, Lung, Kidney or Tonsil.
Cellular Localization	Basement membrane.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Rat
Concentration	Not determined.
Form	Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 0.2% BSA, before the addition of glycerol 40%. Also supplied without BSA and Sodium Azide. See L1225-10X.
Purity	Concentrated antisera
Immunogen	Laminin isolated from a rat yolk sac tumor cell line. Cellular Localization: Basement membrane.
Specificity	Recognizes rat Laminin. Species crossreactivity: Human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.