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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP IH

Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV) and large heparan sulfate proteoglycan (HSPG). All interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.
Catalog #	L1225-15
Applications	Suitable for use in ELISA, Immunoprecipitation, Immunohistochemsitry and Western Blot. Other applications not tested.
Recommended Dilutions	ELISA: 1:20,000-1:100,000
	Western Blot: 1:2000-1:10,000
	Immunohistochemistry: 1:1000-1:5000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with Biotin.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Non reduced human laminin (400kD).
Specificity	Recognizes human laminin. Typically less than 1% crossreactivity against other extracellular matrix proteins was detected by ELISA against purified standards. Negligible crossreactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific crossreaction of anti-laminin antibodies with other human serum proteins or non-laminin extracellular matrix proteins is negligible.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.