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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Laminin (Biotin)

Anti -Laminin (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV) and large heparan sulfate proteoglycan (HSPG). All interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.
Catalog #L1225-15
ApplicationsSuitable for use in ELISA, Immunoprecipitation, Immunohistochemsitry and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:20,000-1:100,000
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with Biotin.
PurityPurified by immunoaffinity chromatography.
ImmunogenNon reduced human laminin (400kD).
SpecificityRecognizes human laminin. Typically less than 1% crossreactivity against other extracellular matrix proteins was detected by ELISA against purified standards. Negligible crossreactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific crossreaction of anti-laminin antibodies with other human serum proteins or non-laminin extracellular matrix proteins is negligible.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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