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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Laminin

Anti -Laminin

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E IH IF RIA
Laminin is a major component of basement membranes and stimulates neurite outgrowth, promotes cell attachment, chemotaxis and cell differentation. Promotes the adhesion and growth of human carcinoma and sarcoma cells, human retinoblastoma cells, liver cells, mouse neuroblastoma cells, mouse embryonal carcinoma cells, and seems to be involved in the development of embryonal tissue.
Catalog #L1225-22
ApplicationsSuitable for use in Immunohistochemistry, Radioimmunoassay, ELISA and Immunofluorescence. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry (Paraffin): 1:500. Use Immunoperoxidase technique on fixed, paraffin-embedded mouse tissues (skin, liver)
ELISA: 1:200 (OD 500)
Immunofluorescence: 1:80 (Frozen mouse skin and liver tissues).
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-40D: IgG, H&L (FITC) Pab Gt x Rb
I1904-40M: IgG, Fc, X-Adsorbed (FITC) Pab Gt x Rb
I1904-41: IgG, X-Adsorbed (FITC) Pab Gt x Rb
I1904-46N: IgG, H&L (FITC) Pab Gt x Rb
Positive ControlMouse skin or liver
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2. No preservatives added.
PurityPurified by affinity chromatography.
ImmunogenLaminin extracted and purified from EHC mouse tumor tissues.
SpecificityRecognizes mouse Laminin. Demonstrates < 0.1% crossreactivity with mouse collagen Types I, II and IV, rat collagen Types I, III and V, rat and human elastin, and mouse fibronectin by RIA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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