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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Laminin

Anti -Laminin

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
Catalog #L1225-24
ApplicationsSuitable for use in Immunoblot, ELISA, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunoblot: 1:2500-1:5000
ELISA: 1:2000-1:4000
Immunohistochemistry: 1:25-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0 with 0.01% Sodium Azide, before the addition of glycerol to 40%.
PurityPurified by immunoaffinity chromatography.
ImmunogenFull length human laminin. Immunization occurred after single-band purity was assessed by SDS-PAGE.
SpecificityRecognizes most mammalian laminins and has negligible crossreactivity with Type I, II, III, IV, V or VI collagens or fibronectin. Non-specific crossreaction of anti-laminin antibodies with other human serum proteins or non-laminin extracellular matrix proteins is negligible. Typically less than 1% crossreactivity against other extracellular matrix proteins was detected by ELISA against purified standards.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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