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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Laminin

Anti -Laminin

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified E B IH IC
Laminin is a large (900kD) complex extracellular glycoprotein and a major component of the basement membrane. Laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin is composed of three different polypeptide chains; alpha, beta and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. Isoforms have been detected for all three chains. The different combinations of the alpha, beta and gamma chains give rise to at least 15 laminin heterotrimers.
Catalog #L1225-27
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:10,000 (0.2ug/ml)
Western Blot: 5-10ug/ml
Immunohistochemistry (Frozen/paraffin): 5-10ug/ml (human stomach carcinoma)
Immunocytochemistry: 5-10ug/ml (HT1080 cells)
Optimal dilutions to be determined by the researcher.
HybridomaP3U1 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3G174
HostMouse
SourceHuman
Concentration~2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 1% BSA. Reconstitute with 50ul sterile ddH2O, 0.1% sodium azide.
PurityPurified by ammonium sulfate precipitation and Ion Exchange chromatography.
ImmunogenHuman Laminin
SpecificityRecognizes the human P2 Laminin fragment.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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