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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IH

Laminins are basement membrane components thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin 5 is a complex glycoprotein composed of three subunits (alpha, beta, and gamma). Laminin 5 is thought to be involved in cell adhesion, signal transduction and differentiation of keratinocytes. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa. Alternatively spliced transcript variants encoding different isoforms have been identified.
Catalog #	L1225-35C
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Immunohistochemistry: Frozen
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~1mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.2, 0.2% sodium azide. Reconstitute with 10ul of sterile ddH2O.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Human Laminin 5; Laminin 5 consists of three chains: alpha 3, beta 3, gamma 2
Specificity	Recognizes human Laminin 5.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.