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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Laminin A

Anti -Laminin A

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B IP IH RIA
Laminin is a protein found in the extracellular matrix, the sheets of protein that form the substrate of all internal organs also called the basement membrane. It is composed of one A chain (400kD) one B1 chain (215kD) and one B2 chain (205kD) all held together by disulfide bonds. The molecule has a cross like form with globular units near the ends of each chain, the sites where it is bound to Collagen IV, heparan sulfate, proteoglycan as well as to the surface of epithelial cells.
Catalog #L1225-40
ApplicationsSuitable for use in ELISA, RIA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry: Suitable for use on paraffin embedded EHS tumor, or tissue frozen in OCT at-70ºC and treated with hyaluronidase.
Optimal dilution determined by the researcher.
SourceAscites
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No2Q599
HostRat
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography
ImmunogenLaminin isolated from mouse EHS (Engelbreth-Holm-Swarm) tumor.
SpecificityRecognizes EHS (Engelbreth-Holm-Swarm) mouse laminin, A chain. Recognizes the carboxy terminal globular domain on the A chain.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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