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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified E B IP IH RIA

Laminin is a protein found in the extracellular matrix, the sheets of protein that form the substrate of all internal organs also called the basement membrane. It is composed of one A chain (400kD) one B1 chain (215kD) and one B2 chain (205kD) all held together by disulfide bonds. The molecule has a cross like form with globular units near the ends of each chain, the sites where it is bound to Collagen IV, heparan sulfate, proteoglycan as well as to the surface of epithelial cells.
Catalog #	L1225-40
Applications	Suitable for use in ELISA, RIA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions	Immunohistochemistry: Suitable for use on paraffin embedded EHS tumor, or tissue frozen in OCT at-70ºC and treated with hyaluronidase.
	Optimal dilution determined by the researcher.
Source	Ascites
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	2Q599
Host	Rat
Source	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Purity	Purified by Protein G affinity chromatography
Immunogen	Laminin isolated from mouse EHS (Engelbreth-Holm-Swarm) tumor.
Specificity	Recognizes EHS (Engelbreth-Holm-Swarm) mouse laminin, A chain. Recognizes the carboxy terminal globular domain on the A chain.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.