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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Laminin A1 (LAMA1)

Anti -Laminin A1 (LAMA1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Laminin subunit Alpha-1 (LAMA1) is a secreted 400kD extracellular matrix glycoprotein that contributes to the formation of basement membrane Laminin isoforms 1 and 3. It is one of three subunits (alpha, beta, and gamma) that interact via their coiled-coil domains to form the approximately 800 kD cruciform, disulfide-linked, Laminin heterotrimer. The 3058 amino acid (aa) residue mature human alpha-1 chain contains an N-terminal Laminin VI domain (aa 18-269), followed by domains V through III containing 17 EGF-like repeats, the coiled-coil domains II and I, and five globular, Laminin G-like domains. Over aa 22-269, human Laminin alpha-1 shares 95% and 91% aa sequence identity with canine and mouse alpha-1 chain, respectively.
Catalog #L1225-46
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Direct ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1ug/ml
Immunohistochemistry: 5-15ug/ml using immersion fixed frozen sectionsof mouse embryo.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by Immunoaffinity chromatography.
ImmunogenRecombinant protein corresponding to aa22-269 from the N-Terminal domain VI of human Laminin alpha 1 (P25391), expressed in E. coli.
SpecificityRecognizes human Laminin alpha 1 N-Terminus Domain VI. Less than 1% crossreactivity was observed with recombinant LAMA4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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