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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified B

Laminins are heterotrimeric glycoproteins and are major components of the basement membrane. Each laminin is comprised of a single , , and chain that heterotrimerize via their coiled-coil domains to form a large cruciform-shaped molecule. Mature Laminin 4 is a subunit of Laminin 411, Laminin 421, and Laminin 423. Mature Laminin 4 is a 1792 amino acid (aa) residue protein that has a Laminin N-terminal domain, four Laminin EGF-like domains, the coiled-coil domain, and five C-terminal tandem Laminin G-like domains. The G-like domains contain binding sites for integrin, heparin, and dystroglycan. Within the region used as immunogen, mouse Laminin 4 shows 91% and 97% aa sequence identity with human and rat Laminin 4, respectively.
Catalog #	L1225-49B
Applications	Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect recombinant mouse Laminin 4. The detection limit for rmLaminin 4 is approximately 10 ng/lane under non-reducing and reducing conditions.
	Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile TBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Mouse
Concentration	As reported
Form	Supplied as a lyophilized powder from a 0.2um filtered solution in PBS containing 50ug of BSA per 1ug of antibody. Reconstitute with sterile TBS, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA, 40-50% glycerol. If 1ml of buffer is used, the concentration will be 50ug/ml.
Purity	Purified by mouse Laminin a4 affinity chromatography and then biotinylated.
Immunogen	NS0-derived rmLaminin a4 (aa826-1816)
Specificity	Selected for use as a detection antibody in mouse Laminin a4 Western Blots.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.