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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Laminin A5

Anti -Laminin A5

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E IP IF
Catalog #L1225-50
ApplicationsSuitable for use in ELISA, Immunofluorescence and Immunoprecipitation. Does not work in Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:11,000 (50% maximal binding to human laminin)
Immunofluorescence: Tissue staining pattern is most consistent with that observed for laminin a5 in mouse.
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No2Q597
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid. No preservative added.
PurityAscites
ImmunogenPurified human laminin
SpecificityRecognizes the globular domain of the a5 chain of intact laminin and blocks the neurite stimulating activity of laminin. This antibody was originally thought to recognize laminin alpha 1 or A, but further characterization has confirmed its specificity for the laminin alpha 5 chain. No crossreactivity is observed to mouse or rat laminin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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