Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

Laminin, a major structural glycoprotein of the basement membranes, is an attachment and survival factor for epithelial cells and can also promote the growth of epithelial cells. Laminin plays an important role in both the structural organization of basement membranes and in the anchorage of cells. Antibodies to laminin chains are useful in basic biology studies as well as in pathological studies concerning, for instance, the integrity of the basement membranes in carcinomas.
Catalog #	L1226-02
Applications	Suitable for use in Immunohistochemistry, Western Blot and other immunoassays for cellular and tumor biology.
Recommended Dilution	Western Blot: 1:500
	Optimal dilutions to be determined by the researcher.
Hybridoma	Derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5G14
Host	Mouse
Source	Human
Concentration	~0.05mg/ml
Form	Supplied as a liquid in PBS, 1% BSA and 0.1% sodium azide, before the addition of glycerol to 40%.
Purity	Purified
Immunogen	Partially pepsin-digested laminins from human placenta.
Specificity	Recognizes the Mr 220kD b1-chain of human laminin. Appears to react with the NH2-terminus of the Ln b1chain. Unlike many other Mabs to Ln b1chain reacts strongly both under reducing and nonreducing conditions. Used to purify Ln-8 (2) and Ln-10 (6) by affinity chromatography.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.