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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Lamin beta (Lamin-B1, LMNB1, LMN2, LMNB)

Anti -Lamin beta (Lamin-B1, LMNB1, LMN2, LMNB)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Lamins are the major components of the nuclear lamina which underlies the nuclear envelope of eukaryotic cells. lamin B is a structural component of the long-sought-after spindle matrix that promotes microtubule assembly and organization in mitosis. Inspection of the deduced amino acid sequence of lamin B revealed the presence in coil 1B of the alpha-helical domain of a leucine heptad repeat region.Lamin B assembled into a matrix-like network in mitosis through a process that depended on the presence of the guanosine triphosphate-bound form of the small guanosine triphosphatase Ran.
Catalog #L1226-05A
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1-0.5ug/ml
Immunohistochemistry (Paraffin): 0.5-1ug/ml. Antigen retrieval by heat is required.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to a sequence from the C-terminal of human Lamin bet. Species Sequence Homology: rat, different by one amino acid; mouse, different by three.
SpecificityRecognizes human Lamin beta. Species Crossreactivity: rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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