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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Laminin B2, gamma1

Anti -Laminin B2, gamma1

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IP IH IF
Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. They are ubiquitously present in basement membrane (BM) along with
entactin/nidogen (EN), collagen type IV (CIV) and large heparan sulfate proteoglycan (HSPG). All interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.
Catalog #L1227-10
ApplicationsSuitable for use in Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunoprecipitation (Native and denatured): 2ug/mg protein lysate using Protein G.
Immunohistochemistry (Acetone-fixed, frozen and acid-alcohol-fixed, paraffin-embedded): 2ug/ml for 30 minutes at RT. Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 minutes at 37°C.
Optimal dilutions to be determined by the researcher.
Positive ControlLS174T cells, normal colon or colon carcinoma.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No0.N.384 (A5)
HostRat
SourceMouse
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide.
Also available without BSA and azide. See L1227-10X.
PurityPurified by Protein G affinity chromatography.
ImmunogenMurine EHS laminin preparation. Cellular Localization: Basement membrane
SpecificityRecognizes the mouse laminin B2/g1 chain at ~210kD. Species Crossreactivity: human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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