| Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. They are ubiquitously present in basement membrane (BM) along with |
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| entactin/nidogen (EN), collagen type IV (CIV) and large heparan sulfate proteoglycan (HSPG). All interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. |
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| Catalog # | L1227-10X |
| Applications | Suitable for use in Immunofluorescence, Immunoprecipitation, and Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilution | Immunoprecipitation (Native and denatured): 2ug/mg protein lysate using Protein G. |
| Immunohistochemistry (acetone-fixed, frozen and acid-alcohol-fixed, paraffin-embedded): 2ug/ml for 30 min at RT. Note: Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 min at 37°C. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Positive Control | LS174T cells, normal colon or colon carcinoma. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a,k |
| Clone No | 0.N.384 (A5) |
| Host | Rat |
| Source | Mouse |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See L1227-10. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Murine EHS laminin preparation. Cellular Localization: Basement membrane. |
| Specificity | Recognizes mouse laminin B2/g1 chain at ~210kD. Species Crossreactivity: human. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
