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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Laminin B2, gamma1

Anti -Laminin B2, gamma1

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH
Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.
Catalog #L1227-13
MW of Antigen ~210kD
Epitope Not determined
Applications Suitable for use in Immunohistochemistry (paraffin sections). Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 min at RT. For staining of formalin-fixed tissues, digest sections with Protease XXV at 1mg/ml PBS for 5 minutes at 37°C
Optimal dilutions to be determined by the researcher.
Positive Control Ls174T cells, Skin.
Cellular Localization Basement membrane.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No3F250
HostMouse
SourceRat
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See L1227-13X
PurityPurified by Protein A affinity chromatography.
ImmunogenRat glomerular basement membrane.
SpecificityRecognizes Laminin B2, gamma1. Species Crossreactivity: human, rat, guinea pig.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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