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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH

Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.
Catalog #	L1227-13
MW of Antigen	~210kD
Epitope	Not determined
Applications	Suitable for use in Immunohistochemistry (paraffin sections). Other applications have not been tested.
Recommended Dilution	Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 min at RT. For staining of formalin-fixed tissues, digest sections with Protease XXV at 1mg/ml PBS for 5 minutes at 37°C
	Optimal dilutions to be determined by the researcher.
Positive Control	Ls174T cells, Skin.
Cellular Localization	Basement membrane.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	3F250
Host	Mouse
Source	Rat
Concentration	~0.2mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See L1227-13X
Purity	Purified by Protein A affinity chromatography.
Immunogen	Rat glomerular basement membrane.
Specificity	Recognizes Laminin B2, gamma1. Species Crossreactivity: human, rat, guinea pig.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.