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Anti -Laminin B2, gamma1 (BSA & Azide Free)
Anti -Laminin B2, gamma1 (BSA & Azide Free)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IH |
|
| Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. | | | Catalog # | L1227-13X | | Applications | Suitable for use in Immunohistochemistry. Other applications have not been tested. | | Recommended Dilution | Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 min at RT. For staining of formalin-fixed tissues, digest sections with Protease XXV at 1mg/ml PBS for 5 minutes at 37°C | | Optimal dilutions to be determined by the researcher | | Positive Control | Ls174T cells, Skin. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a,k | | Clone No | 3F250 | | Host | Mouse | | Source | Rat | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See L1227-13 | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Rat glomerular basement membrane. | | Specificity | Recognizes rat Laminin B2, ~210kSD gamma1. Species Crossreactivity: human, guinea pig. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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