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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -LAP1 (Lamina Associated Polypeptide 1)

Anti -LAP1 (Lamina Associated Polypeptide 1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IP IF
In eukaryotic cells, the nuclear envelope consists of inner and outer membranes which define the border between the nucleus and cytoplasm.
The inner and outer nuclear membranes are joined by large, supramolecular assemblies called nuclear pore complexes through which various molecules pass into and out of the nucleus. The inner membrane is lined by a filamentous meshwork called the nuclear lamina made up of proteins called lamins. The attachment of the lamins to the inner nuclear membrane appears to occur through their association with 4 related integral membrane proteins called Lamin Associated Polypeptides (LAPs). LAP 1A, 1B & 1C share a high degree of sequence homology while LAP 2 is distinct from the others.
LAP 1A & B bind lamins A, C and B1, while LAP 2 binds lamin B1 only.
In addition to lamin binding, LAP 2 has been shown to bind to chromosomes. Phosphorylation of LAP 2 during mitosis inhibits binding to both lamins and chromosomes suggesting that LAP 2 plays an important role in nuclear envelope re-assembly.
Catalog #L1240
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:100 Immunofluorescence staining of LAP 1 in rat liver results in exclusive labeling of the nuclear periphery and exhibits co-localization with lamin staining.
Western Blot: 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4i385
HostMouse
SourceRat
ConcentrationNot determined
FormSupplied as a liquid in PBS with 0.05% sodium azide.
PurityIgG fraction.
ImmunogenPore complex-lamina fraction isolated from rat liver nuclear envelopes.
SpecificityDetects rat lamin associated polypeptide (LAP) 1A, 1B & 1C from rat tissues. Does not crossreact with LAP 2. Detects 3 distinct proteins of molecular weights 75, 68 and 55kD representing LAP 1A, 1B and 1C, respectively in rat liver nuclear envelope fractions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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